rMAP-Myc-Candida surveillance report

Integrated Candida fungal genomics report

This report summarizes paired-end fungal genome analysis for Candida-focused surveillance, combining read QC, Kraken2/Bracken species typing, MEGAHIT assembly, QUAST-only assembly contiguity assessment, and genomic antifungal-resistance screening.

Run generated: 2026-05-30 19:47:45 UTCRun stamp: 20260530_194745_UTCCustom Candida Kraken2/Bracken DBMEGAHIT assembly
Samples analyzed25
Top species groups7
Total AMR hits14
Median N5034,720

1. Executive summary

The primary detected fungal species group was Candidozyma auris. The sample-level cards below provide a compact interpretation of species assignment, QUAST assembly-contiguity metrics, phylogeny status, and genomic AMR screening status.

Interpretation note: genomic antifungal-resistance findings should be treated as screening evidence. Clinically important results should be interpreted with isolate metadata, species identity, validated mutation catalogues, and phenotypic antifungal susceptibility testing where required.

Species distribution

Candidozyma auris
13
Nakaseomyces glabratus
4
Candida albicans
2
Candida tropicalis
2
Clavispora lusitaniae
2
Pichia kudriavzevii
1
Candida parapsilosis
1

2. Surveillance readiness and interpretation dashboard

This integrated table combines species confidence, QUAST assembly-contiguity metrics, AMR marker status, phylogeny eligibility, and optional metadata into a practical surveillance-readiness view.

SampleCollection datesitespeciesSpecies confidenceAssembly QCQUAST contiguity QCAMR screenPhylogenySurveillance statusReason / note
ERR2300788NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300806NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300807NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300808NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300809NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300810NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300812NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300813NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR2300814NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
ERR263535NANACandida albicansHighReviewReview1 marker(s)ExcludedReviewQUAST assembly QC; AMR marker detected
ERR3040852NANAPichia kudriavzeviiLow / reviewReviewReviewNo curated marker detectedExcludedReviewspecies confidence; QUAST assembly QC
ERR331060NANANakaseomyces glabratusLow / reviewPassPassNo curated marker detectedReviewReviewspecies confidence
ERR331061NANANakaseomyces glabratusLow / reviewPassPassNo curated marker detectedReviewReviewspecies confidence
ERR570065NANANakaseomyces glabratusLow / reviewPassPassNo curated marker detectedReviewReviewspecies confidence
SRR10967506NANACandida tropicalisHighPassPassNo curated marker detectedExcludedReady for surveillance interpretationNo major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status
SRR10967518NANACandida tropicalisHighPassPassNo curated marker detectedExcludedReady for surveillance interpretationNo major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status
SRR11092001NANACandidozyma aurisLow / reviewPassPassNo curated marker detectedIncluded / eligibleReviewspecies confidence
SRR11235423NANANakaseomyces glabratusHighPassPass1 marker(s)ReviewReviewAMR marker detected
SRR11235431NANACandida albicansHighReviewReview1 marker(s)ExcludedReviewQUAST assembly QC; AMR marker detected
SRR25474587NANACandida parapsilosisHighReviewReviewNo curated marker detectedExcludedReviewQUAST assembly QC
SRR38908326NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
SRR38908330NANACandidozyma aurisHighPassPass1 marker(s)Included / eligibleReviewAMR marker detected
SRR38908334NANACandidozyma aurisHighPassPassNo curated marker detectedIncluded / eligibleReady for surveillance interpretationNo major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status
SRR6475853NANAClavispora lusitaniaeLow / reviewPassPassNo curated marker detectedExcludedReviewspecies confidence
SRR6476030NANAClavispora lusitaniaeLow / reviewPassPassNo curated marker detectedExcludedReviewspecies confidence

3. Surveillance metadata

No surveillance metadata TSV was provided. Add rMAP_Candida.surveillance_metadata_tsv to your input JSON to display collection date, country/site, specimen type, patient group, facility/ward, and sequencing platform.

4. Sample-level surveillance summary

ERR2300788

Candidozyma auris Review
Species reads99.52%
Contigs670
N5033,153
GC (%)44.95

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300806

Candidozyma auris Review
Species reads98.61%
Contigs688
N5032,749
GC (%)44.95

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300807

Candidozyma auris Review
Species reads98.97%
Contigs659
N5034,720
GC (%)44.95

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300808

Candidozyma auris Review
Species reads98.32%
Contigs677
N5033,038
GC (%)44.95

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300809

Candidozyma auris Review
Species reads98.72%
Contigs695
N5031,632
GC (%)44.93

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300810

Candidozyma auris Review
Species reads99.14%
Contigs688
N5030,447
GC (%)44.93

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300812

Candidozyma auris Review
Species reads99.57%
Contigs653
N5032,986
GC (%)44.95

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300813

Candidozyma auris Review
Species reads99.45%
Contigs687
N5031,998
GC (%)44.94

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR2300814

Candidozyma auris Review
Species reads99.49%
Contigs655
N5033,916
GC (%)44.96

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

ERR263535

Candida albicans Review
Species reads99.44%
Contigs2,777
N5013,483
GC (%)33.86

1 AMR hit(s) Species confidence: High Phylogeny: Excluded

Interpretation: QUAST assembly QC; AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Requires at least 3 samples for a species-specific tree.

ERR3040852

Pichia kudriavzevii Review
Species reads34.89%
Contigs5,146
N5054,792
GC (%)49.64

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Excluded

Interpretation: species confidence; QUAST assembly QC. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Requires at least 3 samples for a species-specific tree.

ERR331060

Nakaseomyces glabratus Review
Species reads73.73%
Contigs427
N5067,770
GC (%)38.08

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Review

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Consensus FASTA lengths differ after reference-based consensus generation.

ERR331061

Nakaseomyces glabratus Review
Species reads73.69%
Contigs433
N5071,482
GC (%)38.08

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Review

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Consensus FASTA lengths differ after reference-based consensus generation.

ERR570065

Nakaseomyces glabratus Review
Species reads73.63%
Contigs430
N5068,055
GC (%)38.08

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Review

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Consensus FASTA lengths differ after reference-based consensus generation.

SRR10967506

Candida tropicalis Ready for surveillance interpretation
Species reads99.86%
Contigs872
N5059,289
GC (%)33.04

No curated genomic AMR marker detected — not susceptible Species confidence: High Phylogeny: Excluded

Interpretation: No major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status. Species note: Top species abundance ≥95%. Phylogeny note: Requires at least 3 samples for a species-specific tree.

SRR10967518

Candida tropicalis Ready for surveillance interpretation
Species reads99.93%
Contigs815
N5066,770
GC (%)33.03

No curated genomic AMR marker detected — not susceptible Species confidence: High Phylogeny: Excluded

Interpretation: No major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status. Species note: Top species abundance ≥95%. Phylogeny note: Requires at least 3 samples for a species-specific tree.

SRR11092001

Candidozyma auris Review
Species reads60.97%
Contigs322
N50118,827
GC (%)47.08

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Included / eligible

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Species group passed phylogeny stage (13 samples)

SRR11235423

Nakaseomyces glabratus Review
Species reads99.99%
Contigs1,264
N5031,653
GC (%)38.83

1 AMR hit(s) Species confidence: High Phylogeny: Review

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Consensus FASTA lengths differ after reference-based consensus generation.

SRR11235431

Candida albicans Review
Species reads99.30%
Contigs4,091
N5012,510
GC (%)33.85

1 AMR hit(s) Species confidence: High Phylogeny: Excluded

Interpretation: QUAST assembly QC; AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Requires at least 3 samples for a species-specific tree.

SRR25474587

Candida parapsilosis Review
Species reads100.00%
Contigs1,392
N5019,980
GC (%)38.61

No curated genomic AMR marker detected — not susceptible Species confidence: High Phylogeny: Excluded

Interpretation: QUAST assembly QC. Species note: Top species abundance ≥95%. Phylogeny note: Skipped by rc172 phylogeny guard because this detected species is not among the defined Candida species with an exported reference FASTA. Valid species with references are still processed.

SRR38908326

Candidozyma auris Review
Species reads99.37%
Contigs115
N50209,202
GC (%)45.14

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

SRR38908330

Candidozyma auris Review
Species reads99.25%
Contigs117
N50270,319
GC (%)45.14

1 AMR hit(s) Species confidence: High Phylogeny: Included / eligible

Interpretation: AMR marker detected. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

SRR38908334

Candidozyma auris Ready for surveillance interpretation
Species reads99.16%
Contigs146
N50144,269
GC (%)45.15

No curated genomic AMR marker detected — not susceptible Species confidence: High Phylogeny: Included / eligible

Interpretation: No major automated warning flags using species typing, QUAST assembly QC, AMR screening, and phylogeny status. Species note: Top species abundance ≥95%. Phylogeny note: Species group passed phylogeny stage (13 samples)

SRR6475853

Clavispora lusitaniae Review
Species reads55.62%
Contigs54
N50609,454
GC (%)45.52

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Excluded

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Requires at least 3 samples for a species-specific tree.

SRR6476030

Clavispora lusitaniae Review
Species reads54.11%
Contigs161
N50240,220
GC (%)46.15

No curated genomic AMR marker detected — not susceptible Species confidence: Low / review Phylogeny: Excluded

Interpretation: species confidence. Species note: Top species abundance <80%; possible mixed sample, contamination, or weak species assignment. Phylogeny note: Requires at least 3 samples for a species-specific tree.

5. Candida species typing using Kraken2/Bracken

Top species calls are derived from the custom Candida-focused Kraken2/Bracken database bundled in the species-typing Docker image.

SampleTop speciesReads (%)clade_readstaxon_readstaxidevidence
ERR2300788Candidozyma auris
99.52%
5,062,1655,062,165498019Bracken species-level abundance
ERR2300806Candidozyma auris
98.61%
5,042,0955,042,095498019Bracken species-level abundance
ERR2300807Candidozyma auris
98.97%
5,231,0795,231,079498019Bracken species-level abundance
ERR2300808Candidozyma auris
98.32%
5,035,0325,035,032498019Bracken species-level abundance
ERR2300809Candidozyma auris
98.72%
6,247,8746,247,874498019Bracken species-level abundance
ERR2300810Candidozyma auris
99.14%
6,041,3706,041,370498019Bracken species-level abundance
ERR2300812Candidozyma auris
99.57%
5,103,0875,103,087498019Bracken species-level abundance
ERR2300813Candidozyma auris
99.45%
4,994,3074,994,307498019Bracken species-level abundance
ERR2300814Candidozyma auris
99.49%
5,038,9515,038,951498019Bracken species-level abundance
ERR263535Candida albicans
99.44%
1,749,3311,749,3315476Bracken species-level abundance
ERR3040852Pichia kudriavzevii
34.89%
6,6696,6694909Bracken species-level abundance
ERR331060Nakaseomyces glabratus
73.73%
190,301190,3015478Bracken species-level abundance
ERR331061Nakaseomyces glabratus
73.69%
178,423178,4235478Bracken species-level abundance
ERR570065Nakaseomyces glabratus
73.63%
154,728154,7285478Bracken species-level abundance
SRR10967506Candida tropicalis
99.86%
2,260,4512,260,4515482Bracken species-level abundance
SRR10967518Candida tropicalis
99.93%
2,129,0992,129,0995482Bracken species-level abundance
SRR11092001Candidozyma auris
60.97%
42,72942,729498019Bracken species-level abundance
SRR11235423Nakaseomyces glabratus
99.99%
1,676,7911,676,7915478Bracken species-level abundance
SRR11235431Candida albicans
99.30%
2,986,2922,986,2925476Bracken species-level abundance
SRR25474587Candida parapsilosis
100.00%
1,217,5561,217,5565480Bracken species-level abundance
SRR38908326Candidozyma auris
99.37%
1,244,4451,244,445498019Bracken species-level abundance
SRR38908330Candidozyma auris
99.25%
1,287,4291,287,429498019Bracken species-level abundance
SRR38908334Candidozyma auris
99.16%
1,052,4731,052,473498019Bracken species-level abundance
SRR6475853Clavispora lusitaniae
55.62%
19,64219,64236911Bracken species-level abundance
SRR6476030Clavispora lusitaniae
54.11%
45,00145,00136911Bracken species-level abundance

6. MEGAHIT assembly summary

SamplecontigsTotal bpN50Largest contig
ERR230078867012,407,99233,153126,993
ERR230080668812,394,99532,749140,237
ERR230080765912,407,02134,720127,023
ERR230080867712,409,56633,038142,154
ERR230080969512,419,10131,632106,491
ERR230081068812,414,15230,447174,120
ERR230081265312,407,00232,986115,417
ERR230081368712,410,99331,998133,252
ERR230081465512,403,23633,916129,535
ERR2635352,77713,941,32113,483132,505
ERR30408525,14613,603,31554,792367,079
ERR33106042711,615,43467,770166,550
ERR33106143311,618,74071,482269,717
ERR57006543011,617,89568,055229,603
SRR1096750687214,597,39659,289262,902
SRR1096751881514,557,60466,770251,077
SRR1109200132212,796,469118,827423,624
SRR112354231,26412,445,09631,653116,487
SRR112354314,09115,459,06712,51088,610
SRR254745871,39213,028,12019,980105,575
SRR3890832611512,333,174209,202876,588
SRR3890833011712,343,577270,319781,411
SRR3890833414612,262,768144,269441,195
SRR6475853549,654,719609,4541,021,854
SRR647603016111,704,652240,220598,301

7. Assembly quality assessment with QUAST

QUAST values are parsed from the native per-sample report.tsv format and transposed into one row per sample. This default surveillance mode reports QUAST assembly contiguity metrics such as contig count, total length, N50, largest contig, and GC percentage. It is very fast. BUSCO and Compleasm tasks are available as optional modules, but they are disabled by default in the recommended JSON and are not required for this QUAST-based assembly assessment.

Sample# contigsLargest contigTotal lengthGC (%)N50
ERR2300788670126,99312,407,99244.9533,153
ERR2300806688140,23712,394,99544.9532,749
ERR2300807659127,02312,407,02144.9534,720
ERR2300808677142,15412,409,56644.9533,038
ERR2300809695106,49112,419,10144.9331,632
ERR2300810688174,12012,414,15244.9330,447
ERR2300812653115,41712,407,00244.9532,986
ERR2300813687133,25212,410,99344.9431,998
ERR2300814655129,53512,403,23644.9633,916
ERR2635352,777132,50513,941,32133.8613,483
ERR30408525,146367,07913,603,31549.6454,792
ERR331060427166,55011,615,43438.0867,770
ERR331061433269,71711,618,74038.0871,482
ERR570065430229,60311,617,89538.0868,055
SRR10967506872262,90214,597,39633.0459,289
SRR10967518815251,07714,557,60433.0366,770
SRR11092001322423,62412,796,46947.08118,827
SRR112354231,264116,48712,445,09638.8331,653
SRR112354314,09188,61015,459,06733.8512,510
SRR254745871,392105,57513,028,12038.6119,980
SRR38908326115876,58812,333,17445.14209,202
SRR38908330117781,41112,343,57745.14270,319
SRR38908334146441,19512,262,76845.15144,269
SRR6475853541,021,8549,654,71945.52609,454
SRR6476030161598,30111,704,65246.15240,220

8. Fungal antifungal-resistance characterization

This section reports mutation/gene-level evidence emitted by the configured fungal AMR container. A “No marker detected” result is not a susceptible call. Fluconazole resistance can be caused by ERG11 alterations, TAC1/UPC2/MRR1/PDR1-mediated efflux, aneuploidy/LOH, copy-number changes, species-specific mechanisms, or markers absent from the current AMR database.

Samplespeciesdrug_classdrugGene / statusmutationeffectevidence_levelinterpretation
ERR2300788Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300788.ChroQueTas/ERR2300788.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300806Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300806.ChroQueTas/ERR2300806.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300807Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300807.ChroQueTas/ERR2300807.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300808Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300808.ChroQueTas/ERR2300808.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300809Candidozyma aurisazolefluconazole/other azolesCyp51V125AFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 V125A (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(NA/-2),Itraconazole(NA/-2),Posaconazole(NA/-2),Voriconazole(NA/-2). Source: amr_out/ERR2300809.ChroQueTas/ERR2300809.contigs.ChroQueTaS.Cyp51.1.tsv:125
ERR2300810Candidozyma aurisazolefluconazole/other azolesCyp51V125AFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 V125A (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(NA/-2),Itraconazole(NA/-2),Posaconazole(NA/-2),Voriconazole(NA/-2). Source: amr_out/ERR2300810.ChroQueTas/ERR2300810.contigs.ChroQueTaS.Cyp51.1.tsv:125
ERR2300812Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300812.ChroQueTas/ERR2300812.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300813Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300813.ChroQueTas/ERR2300813.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR2300814Candidozyma aurisazole/echinocandin/polyene/flucytosinefluconazole/other azoles/echinocandins/amphotericin_B/flucytosineCyp51Y132FFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 Y132F (FungAMR MUTATION). Drug evidence from ChroQueTas: 5-fluorocytosine(NA/-8),Amphotericin_B(8/-8),Anidulafungin(NA/-8),Caspofungin(8/-8),Fluconazole(2/-8),Itraconazole(8/-8),Micafungin(NA/-8),Posaconazole(8/-8),Voriconazole(2/-8). Source: amr_out/ERR2300814.ChroQueTas/ERR2300814.contigs.ChroQueTaS.Cyp51.1.tsv:132
ERR263535Candida albicansazolefluconazole/other azolesCyp51E266DFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 E266D (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(NA/-1). Source: amr_out/ERR263535.ChroQueTas/ERR263535.contigs.ChroQueTaS.Cyp51.1.tsv:266
ERR3040852Pichia kudriavzeviiazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/ERR3040852.ChroQueTas.AMR_summary.tsv No AMR mutations found in ERR3040852.contigs
ERR331060Nakaseomyces glabratusazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/ERR331060.ChroQueTas.AMR_summary.tsv No AMR mutations found in ERR331060.contigs
ERR331061Nakaseomyces glabratusazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/ERR331061.ChroQueTas.AMR_summary.tsv No AMR mutations found in ERR331061.contigs
ERR570065Nakaseomyces glabratusazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/ERR570065.ChroQueTas.AMR_summary.tsv No AMR mutations found in ERR570065.contigs
SRR10967506Candida tropicalisazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR10967506.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR10967506.contigs
SRR10967518Candida tropicalisazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR10967518.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR10967518.contigs
SRR11092001Candidozyma aurisazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR11092001.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR11092001.contigs
SRR11235423Nakaseomyces glabratusantifungalspecies-aware antifungal panelBen1V254IFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Ben1 V254I (FungAMR MUTATION). Drug evidence from ChroQueTas: Various(NA/-8). Source: amr_out/SRR11235423.ChroQueTas/SRR11235423.contigs.ChroQueTaS.Ben1.1.tsv:254
SRR11235431Candida albicansazolefluconazole/other azolesCyp51E266DFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 E266D (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(NA/-1). Source: amr_out/SRR11235431.ChroQueTas/SRR11235431.contigs.ChroQueTaS.Cyp51.1.tsv:266
SRR25474587Candida parapsilosisazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR25474587.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR25474587.contigs
SRR38908326Candidozyma aurisazolefluconazole/other azolesCyp51V125AFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 V125A (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(NA/-2),Itraconazole(NA/-2),Posaconazole(NA/-2),Voriconazole(NA/-2). Source: amr_out/SRR38908326.ChroQueTas/SRR38908326.contigs.ChroQueTaS.Cyp51.1.tsv:125
SRR38908330Candidozyma aurisazolefluconazole/other azolesCyp51K143RFungAMR MUTATIONFungAMR curated marker detected by ChroQueTasCurated ChroQueTas/FungAMR marker detected: Cyp51 K143R (FungAMR MUTATION). Drug evidence from ChroQueTas: Fluconazole(2/NA),Voriconazole(2/NA). Source: amr_out/SRR38908330.ChroQueTas/SRR38908330.contigs.ChroQueTaS.Cyp51.1.tsv:143
SRR38908334Candidozyma aurisazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR38908334.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR38908334.contigs
SRR6475853Clavispora lusitaniaeazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR6475853.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR6475853.contigs
SRR6476030Clavispora lusitaniaeazole/otherfluconazole/otherNo marker — not susceptibleNANAscanner_detected_candidate_markerThe AMR scanner produced text consistent with a possible curated resistance marker, but rc151 could not parse an exact ChroQueTas marker row. Review fungal_amr.raw.tsv and fungal_amr.log for the exact record: amr_out/SRR6476030.ChroQueTas.AMR_summary.tsv No AMR mutations found in SRR6476030.contigs

9. Species-aware core-SNP phylogeny

When enabled, rMAP-Candida builds phylogenies separately for each species with sufficient samples and a matching reference. Mixed-species phylogenies are intentionally avoided. Outputs include species-group summaries, core-SNP alignments, and IQ-TREE Newick trees.

SpeciesStatusSamplesVariant-calling branchPloidy modelCore variable sitesNotes
Candida parapsilosisSKIPPED_SPECIES_NOT_IN_REFERENCE_MANIFEST1NANANASkipped by rc172 phylogeny guard because this detected species is not among the defined Candida species with an exported reference FASTA. Valid species with references are still processed.
Candida albicansSKIPPED_TOO_FEW_SAMPLES2snippy_core1NARequires at least 3 samples for a species-specific tree.
Candida tropicalisSKIPPED_TOO_FEW_SAMPLES2snippy_core1NARequires at least 3 samples for a species-specific tree.
Candidozyma aurisPASS13snippy_core_rc170_tb_styleNA27811rc170 used the rMAP-TB-style Snippy -> snippy-core branch. Species grouping was based on Kraken2/Bracken top-species calls; mixed-species trees are intentionally avoided. Recombination is not explicitly filtered.
Clavispora lusitaniaeSKIPPED_TOO_FEW_SAMPLES2diploid_aware_bcftools_iupac_consensus2NARequires at least 3 samples for a species-specific tree.
Nakaseomyces glabratusFAILED_CORE_SNP_ALIGNMENT4haploid_bcftools_consensus1NAConsensus FASTA lengths differ after reference-based consensus generation.
Pichia kudriavzeviiSKIPPED_TOO_FEW_SAMPLES1diploid_aware_bcftools_iupac_consensus2NARequires at least 3 samples for a species-specific tree.

Candidozyma auris

Candidozyma auris core-SNP phylogenetic tree
Species without a displayed tree:
  • Candida parapsilosis: SKIPPED_SPECIES_NOT_IN_REFERENCE_MANIFEST; samples after species-specific consensus/eligibility = 1; branch = NA; note = Skipped by rc172 phylogeny guard because this detected species is not among the defined Candida species with an exported reference FASTA. Valid species with references are still processed.
  • Candida albicans: SKIPPED_TOO_FEW_SAMPLES; samples after species-specific consensus/eligibility = 2; branch = snippy_core; note = Requires at least 3 samples for a species-specific tree.
  • Candida tropicalis: SKIPPED_TOO_FEW_SAMPLES; samples after species-specific consensus/eligibility = 2; branch = snippy_core; note = Requires at least 3 samples for a species-specific tree.
  • Clavispora lusitaniae: SKIPPED_TOO_FEW_SAMPLES; samples after species-specific consensus/eligibility = 2; branch = diploid_aware_bcftools_iupac_consensus; note = Requires at least 3 samples for a species-specific tree.
  • Nakaseomyces glabratus: FAILED_CORE_SNP_ALIGNMENT; samples after species-specific consensus/eligibility = 4; branch = haploid_bcftools_consensus; note = Consensus FASTA lengths differ after reference-based consensus generation.
  • Pichia kudriavzevii: SKIPPED_TOO_FEW_SAMPLES; samples after species-specific consensus/eligibility = 1; branch = diploid_aware_bcftools_iupac_consensus; note = Requires at least 3 samples for a species-specific tree.
Phylogeny interpretation note: rMAP-Candida builds one tree per species. Mixed-species Candida phylogenies are intentionally avoided. Treat these trees as species-level genomic relatedness visualizations unless recombination filtering and epidemiologic metadata support transmission interpretation.

10. Species-aware pairwise SNP distances and closest-neighbor summary

Closest-neighbor summary

speciesSampleClosest sampleSNP distanceConservative cluster flag
Candidozyma aurisERR2300788ERR23008060Possible close genetic relationship
Candidozyma aurisERR2300806ERR23007880Possible close genetic relationship
Candidozyma aurisERR2300807ERR23007880Possible close genetic relationship
Candidozyma aurisERR2300808ERR23007880Possible close genetic relationship
Candidozyma aurisERR2300809ERR23008100Possible close genetic relationship
Candidozyma aurisERR2300810ERR23008090Possible close genetic relationship
Candidozyma aurisERR2300812ERR23007880Possible close genetic relationship
Candidozyma aurisERR2300813ERR23007880Possible close genetic relationship
Candidozyma aurisERR2300814ERR23007880Possible close genetic relationship
Candidozyma aurisSRR11092001ERR230078826797Distinct / review with metadata
Candidozyma aurisSRR38908326ERR23008092Possible close genetic relationship
Candidozyma aurisSRR38908330ERR23007880Possible close genetic relationship
Candidozyma aurisSRR38908334ERR23007881565Distinct / review with metadata

Pairwise SNP-distance matrix/table

speciesSample ASample BSNP distanceCompared core-SNP sites
Candidozyma aurisERR2300788ERR2300806027811
Candidozyma aurisERR2300788ERR2300807027811
Candidozyma aurisERR2300788ERR2300808027811
Candidozyma aurisERR2300788ERR230080938027811
Candidozyma aurisERR2300788ERR230081038027811
Candidozyma aurisERR2300788ERR2300812027811
Candidozyma aurisERR2300788ERR2300813027811
Candidozyma aurisERR2300788ERR2300814027811
Candidozyma aurisERR2300788SRR110920012679727811
Candidozyma aurisERR2300788SRR3890832638227811
Candidozyma aurisERR2300788SRR38908330027811
Candidozyma aurisERR2300788SRR38908334156527811
Candidozyma aurisERR2300806ERR2300807027811
Candidozyma aurisERR2300806ERR2300808027811
Candidozyma aurisERR2300806ERR230080938027811
Candidozyma aurisERR2300806ERR230081038027811
Candidozyma aurisERR2300806ERR2300812027811
Candidozyma aurisERR2300806ERR2300813027811
Candidozyma aurisERR2300806ERR2300814027811
Candidozyma aurisERR2300806SRR110920012679727811
Candidozyma aurisERR2300806SRR3890832638227811
Candidozyma aurisERR2300806SRR38908330027811
Candidozyma aurisERR2300806SRR38908334156527811
Candidozyma aurisERR2300807ERR2300808027811
Candidozyma aurisERR2300807ERR230080938027811
Candidozyma aurisERR2300807ERR230081038027811
Candidozyma aurisERR2300807ERR2300812027811
Candidozyma aurisERR2300807ERR2300813027811
Candidozyma aurisERR2300807ERR2300814027811
Candidozyma aurisERR2300807SRR110920012679727811
Candidozyma aurisERR2300807SRR3890832638227811
Candidozyma aurisERR2300807SRR38908330027811
Candidozyma aurisERR2300807SRR38908334156527811
Candidozyma aurisERR2300808ERR230080938027811
Candidozyma aurisERR2300808ERR230081038027811
Candidozyma aurisERR2300808ERR2300812027811
Candidozyma aurisERR2300808ERR2300813027811
Candidozyma aurisERR2300808ERR2300814027811
Candidozyma aurisERR2300808SRR110920012679727811
Candidozyma aurisERR2300808SRR3890832638227811
Candidozyma aurisERR2300808SRR38908330027811
Candidozyma aurisERR2300808SRR38908334156527811
Candidozyma aurisERR2300809ERR2300810027811
Candidozyma aurisERR2300809ERR230081238027811
Candidozyma aurisERR2300809ERR230081338027811
Candidozyma aurisERR2300809ERR230081438027811
Candidozyma aurisERR2300809SRR110920012683227811
Candidozyma aurisERR2300809SRR38908326227811
Candidozyma aurisERR2300809SRR3890833038027811
Candidozyma aurisERR2300809SRR38908334158827811
Candidozyma aurisERR2300810ERR230081238027811
Candidozyma aurisERR2300810ERR230081338027811
Candidozyma aurisERR2300810ERR230081438027811
Candidozyma aurisERR2300810SRR110920012683227811
Candidozyma aurisERR2300810SRR38908326227811
Candidozyma aurisERR2300810SRR3890833038027811
Candidozyma aurisERR2300810SRR38908334158827811
Candidozyma aurisERR2300812ERR2300813027811
Candidozyma aurisERR2300812ERR2300814027811
Candidozyma aurisERR2300812SRR110920012679727811
Candidozyma aurisERR2300812SRR3890832638227811
Candidozyma aurisERR2300812SRR38908330027811
Candidozyma aurisERR2300812SRR38908334156527811
Candidozyma aurisERR2300813ERR2300814027811
Candidozyma aurisERR2300813SRR110920012679727811
Candidozyma aurisERR2300813SRR3890832638227811
Candidozyma aurisERR2300813SRR38908330027811
Candidozyma aurisERR2300813SRR38908334156527811
Candidozyma aurisERR2300814SRR110920012679727811
Candidozyma aurisERR2300814SRR3890832638227811
Candidozyma aurisERR2300814SRR38908330027811
Candidozyma aurisERR2300814SRR38908334156527811
Candidozyma aurisSRR11092001SRR389083262683227811
Candidozyma aurisSRR11092001SRR389083302679727811
Candidozyma aurisSRR11092001SRR389083342687127811
Candidozyma aurisSRR38908326SRR3890833038227811
Candidozyma aurisSRR38908326SRR38908334159027811
Candidozyma aurisSRR38908330SRR38908334156527811
SNP-distance interpretation: low SNP distances suggest close genetic relatedness but should not be interpreted as transmission without epidemiological metadata, recombination-aware analysis, and species-specific validation.

11. Output navigation and provenance

The workflow emits downloadable tabular outputs in addition to this integrated HTML report.

rMAP-Myc-Candida-Candida_summary.tsv
rMAP_Candida_surveillance_summary.tsv
rMAP_Candida_pairwise_snp_distances.tsv